Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice

被引：51

作者：

Zhu, Huachen ^{[1
,2
,3
]}

Wang, Jia ^{[1
,2
,3
]}

Wang, Pui ^{[1
,2
,3
]}

Song, Wenjun ^{[1
,2
,3
]}

Zheng, Zuoyi ^{[3
]}

Chen, Rirong ^{[3
]}

Guo, Kunyuan ^{[3
]}

Zhang, Taixing ^{[3
]}

Peiris, Joseph S. M. ^{[1
,2
]}

Chen, Honglin ^{[1
,2
,3
]}

Guan, Yi ^{[1
,2
,3
]}

机构：

[1] Univ Hong Kong, Li Ka Shing Fac Med, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China

[2] Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[3] Shantou Univ, Coll Med, Int Inst Infect & Immun, Shantou 515031, Guangdong, Peoples R China

来源：

VIROLOGY | 2010年 / 401卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Influenza virus; Pathogenicity; Virulence; PB2; 627; residue; Temperature-sensitivity; INFLUENZA-A VIRUSES; HOST-RANGE; MOLECULAR-BASIS; AMINO-ACID; POLYMERASE; REPLICATION; DETERMINANT; ADAPTATION; CELLS;

D O I：

10.1016/j.virol.2010.02.024

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6 months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus. (C) 2010 Elsevier Inc. All rights reserved.

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页数：5

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