C2C12 myoblasts release micro-vesicles containing mtDNA and proteins involved in signal transduction

被引：238

作者：

Guescini, M. ^{[1
]}

Guidolin, D. ^{[2
]}

Vallorani, L. ^{[1
]}

Casadei, L. ^{[1
]}

Gioacchini, A. M. ^{[1
]}

Tibollo, P. ^{[1
]}

Battistelli, M. ^{[3
]}

Falcieri, E. ^{[3
,4
]}

Battistin, L. ^{[5
]}

Agnati, L. F. ^{[5
]}

Stocchi, V. ^{[1
]}

机构：

[1] Univ Urbino Carlo Bo, Dept Biomol Sci, I-61029 Urbino, Italy

[2] Univ Padua, Dept Anat & Physiol, I-35100 Padua, Italy

[3] Univ Urbino Carlo Bo, Dept Human Environm & Nat Sci, I-61029 Urbino, Italy

[4] Ist Ortoped Rizzoli, CNR, Inst Mol Genet, I-40136 Bologna, Italy

[5] IRCCS San Camillo Lido, I-30100 Venice, Italy

来源：

EXPERIMENTAL CELL RESEARCH | 2010年 / 316卷 / 12期

关键词：

Myoblasts; Exosomes; Mitochondrial DNA; Inter-cellular communication; Bioinformatics analysis; HUMAN SKELETAL-MUSCLE; MITOCHONDRIAL-DNA; ENDOCRINE ORGAN; HOT-SPOTS; EXOSOMES; EXPRESSION; EXERCISE; IL-6; AGGREGATION; PREDICTION;

D O I：

10.1016/j.yexcr.2010.04.006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Micro-vesicles can be released by different cell types and operate as 'safe containers' mediating inter-cellular communication In this work we investigated whether cultured myoblasts could release exosomes. The reported data demonstrate, for the first time, that C2C12 myoblasts release micro-vesicles as shown by the presence of two exosome markers (Tsg101 and Alix proteins) Using real-time PCR analysis it was shown that these micro-vesicles, like other cell types, carry mtDNA. Proteomic characterization of the released micro-vesicle contents showed the presence of many proteins involved in signal transduction The bioinformatics assessment of the Disorder Index and Aggregation Index of these proteins suggested that C2C12 micro-vesicles mainly deliver the machinery for signal transduction to target cells rather than key proteins involved in hub functions in molecular networks The presence of IGFBP-5 in the purified micro-vesicles represents an exception, since this binding protein can play a key role in the modulation of the IGF-1 signalling pathway. In conclusion, the present findings demonstrate that skeletal muscle cells release micro-vesicles, which probably have an important role in the communication processes within skeletal muscles and between skeletal muscles and other organs In particular, the present findings suggest possible new diagnostic approaches to skeletal muscle diseases. (C) 2010 Elsevier Inc All rights reserved

引用

页码：1977 / 1984

页数：8

共 49 条

[1] Structural plasticity in G-protein coupled receptors as demonstrated by the allosteric actions of homocysteine and computer-assisted analysis of disordered domains [J].