Mitochondrial DNA analysis of ancient human teeth from a XVIth century archeological excavation

被引：2

作者：

Hernandez, A ^{[1
]}

Mamely, I ^{[1
]}

Sanchez, JJ ^{[1
]}

Cuellas, JA ^{[1
]}

Alonso, A ^{[1
]}

Rodriguez, C ^{[1
]}

机构：

[1] Inst Nacl Toxicol, Tenerife 38320, Spain

来源：

PROGRESS IN FORENSIC GENETICS 9 | 2003年 / 1239卷

关键词：

ancient DNA; mitochondrial DNA; sequencing;

D O I：

10.1016/S0531-5131(02)00223-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Our laboratories are involved in a large-scale study investigating the different hypotheses about the founding Canary Islands populations-making use of physical-anthropological, genetic and archeological approaches. The main problems associated with ancient DNA studies are low yield, high fragmentation and contamination with exogenous DNA. In this study, we tried to evaluate the viability of our laboratory set-up to accomplish mitochondrial DNA (mtDNA) isolation and analysis from ancient samples. We present preliminary results of HVI region mtDNA sequencing of several teeth from the Monastery of Los Silos (North of Tenerife Island), an archeological excavation site dating back to the XVIth century, used as a graveyard for some time. We also make some considerations about the amplification yield by comparing two different PCR strategies. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：601 / 604

页数：4

共 6 条

[1] Multiplex-PCR of short amplicons for mtDNA sequencing from ancient DNA [J].