Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing

被引：47

作者：

Haanperä, M

Huovinen, P

Jalava, J

机构：

[1] Natl Publ Hlth Inst, Human Microbial Ecol Lab, FI-20520 Turku, Finland

[2] Natl Publ Hlth Inst, Antimicrobial Res Lab, FI-20520 Turku, Finland

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2005年 / 49卷 / 01期

关键词：

D O I：

10.1128/AAC.49.1.457-460.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A pyrosequencing method for detection and quantification of macrolide resistance mutations at positions 2058 and 2059 (Escherichia coli numbering) of the 23S rRNA gene is described. The method was developed and tested for Streptococcus pneumoniae, Streptococcus pyogenes, Mycobacterium avium, Campylobacter jejuni, and Haemophilus influenzae.

引用

页码：457 / 460

页数：4

共 14 条

[11] Mutations in 23S rRNA and ribosomal protein L4 account for resistance in pneumococcal strains selected in vitro by macrolide passage [J].