EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.)

被引:36
作者
Studer, Bruno [1 ]
Kolliker, Roland [2 ]
Muylle, Hilde [3 ]
Asp, Torben [1 ]
Frei, Ursula
Roldan-Ruiz, Isabel [3 ]
Barre, Philippe [4 ]
Tomaszewski, Celine [5 ]
Meally, Helena [5 ]
Barth, Susanne [5 ]
Skot, Leif [6 ]
Armstead, Ian P. [6 ]
Dolstra, Oene [7 ]
Luebberstedt, Thomas [8 ]
机构
[1] Aarhus Univ, Dept Genet & Biotechnol, Fac Agr Sci, Res Ctr Flakkebjerg, DK-4200 Slagelse, Denmark
[2] Res Stn ART, CH-8046 Zurich, Switzerland
[3] Inst Agr & Fisheries Res ILVO, Plant Sci Unit Growth & Dev, B-9090 Melle, Belgium
[4] INRA, Unite Rech Pluridisciplinaire Prairies & Plantes, F-86600 Lusignan, France
[5] TEAGASC, Crops Res Ctr Oak Pk, Carlow, Ireland
[6] Aberystwyth Univ, IBERS, Aberystwyth SY23 3EB, Ceredigion, Wales
[7] Wageningen Univ & Res Ctr PRI, Wageningen UR Plant Breeding, NL-6700 AA Wageningen, Netherlands
[8] Iowa State Univ, Dept Agron, Ames, IA 50011 USA
来源
BMC PLANT BIOLOGY | 2010年 / 10卷
关键词
CROWN RUST RESISTANCE; F-SP LOLII; SEQUENCE REPEAT MARKERS; QUANTITATIVE TRAIT LOCI; PERENNIAL RYEGRASS; MULTIFLORUM LAM; QTL ANALYSIS; MICROSATELLITE MARKERS; MAPPING POPULATIONS; AFLP MARKERS;
D O I
10.1186/1471-2229-10-177
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage maps of these populations have been aligned with each other using publicly available DNA markers, the number of common markers among genetic maps is still low, limiting the ability to compare candidate gene and QTL locations across germplasm. Results: A set of 204 expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers has been assigned to map positions using eight different ryegrass mapping populations. Marker properties of a subset of 64 EST-SSRs were assessed in six to eight individuals of each mapping population and revealed 83% of the markers to be polymorphic in at least one population and an average number of alleles of 4.88. EST-SSR markers polymorphic in multiple populations served as anchor markers and allowed the construction of the first comprehensive consensus map for ryegrass. The integrated map was complemented with 97 SSRs from previously published linkage maps and finally contained 284 EST-derived and genomic SSR markers. The total map length was 742 centiMorgan (cM), ranging for individual chromosomes from 70 cM of linkage group (LG) 6 to 171 cM of LG 2. Conclusions: The consensus linkage map for ryegrass based on eight mapping populations and constructed using a large set of publicly available Lolium EST-SSRs mapped for the first time together with previously mapped SSR markers will allow for consolidating existing mapping and QTL information in ryegrass. Map and markers presented here will prove to be an asset in the development for both molecular breeding of ryegrass as well as comparative genetics and genomics within grass species.
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页数:10
相关论文
共 58 条
[1]   Segregation distortion in Lolium:: evidence for genetic effects [J].
Anhalt, U. C. M. ;
Heslop-Harrison, P. ;
Byrne, S. ;
Guillard, A. ;
Barth, S. .
THEORETICAL AND APPLIED GENETICS, 2008, 117 (02) :297-306
[2]   Quantitative trait loci mapping for biomass yield traits in a Lolium inbred line derived F2 population [J].
Anhalt, U. C. M. ;
Heslop-Harrison , J. S. ;
Piepho, H. P. ;
Byrne, S. ;
Barth, S. .
EUPHYTICA, 2009, 170 (1-2) :99-107
[3]   Identifying genetic components controlling fertility in the outcrossing grass species perennial ryegrass (Lolium perenne) by quantitative trait loci analysis and comparative genetics [J].
Armstead, I. P. ;
Turner, L. B. ;
Marshall, A. H. ;
Humphreys, M. O. ;
King, I. P. ;
Thorogood, D. .
NEW PHYTOLOGIST, 2008, 178 (03) :559-571
[4]   Synteny between a major heading-date QTL in perennial ryegrass (Lolium perenne L.) and the Hd3 heading-date locus in rice [J].
Armstead, IP ;
Turner, LB ;
Farrell, M ;
Skot, L ;
Gomez, P ;
Montoya, T ;
Donnison, IS ;
King, IP ;
Humphreys, MO .
THEORETICAL AND APPLIED GENETICS, 2004, 108 (05) :822-828
[5]   Comparison and integration of genetic maps generated from F2 and BC1-type mapping populations in perennial ryegrass [J].
Armstead, IP ;
Turner, LB ;
King, IP ;
Cairns, AJ ;
Humphreys, MO .
PLANT BREEDING, 2002, 121 (06) :501-507
[6]   Quantitative trait loci for leaf length in perennial ryegrass (Lolium perenne L.) [J].
Barre, P. ;
Moreau, L. ;
Mi, F. ;
Turner, L. ;
Gastal, F. ;
Julier, B. ;
Ghesquiere, M. .
GRASS AND FORAGE SCIENCE, 2009, 64 (03) :310-321
[7]  
Barre P, 2000, P 2 INT S MOL BREED, P60
[8]   A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers [J].
Bert, PF ;
Charmet, G ;
Sourdille, P ;
Hayward, MD ;
Balfourier, F .
THEORETICAL AND APPLIED GENETICS, 1999, 99 (3-4) :445-452
[9]   M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods [J].
Boutin-Ganache, I ;
Raposo, M ;
Raymond, M ;
Deschepper, CF .
BIOTECHNIQUES, 2001, 31 (01) :24-+
[10]   QTL analysis and comparative genomics of herbage quality traits in perennial ryegrass (Lolium perenne L.) [J].
Cogan, N ;
Smith, K ;
Yamada, T ;
Francki, M ;
Vecchies, A ;
Jones, E ;
Spangenberg, G ;
Forster, J .
THEORETICAL AND APPLIED GENETICS, 2005, 110 (02) :364-380