Different characteristics of rat retinal progenitor cells from different culture periods

被引：42

作者：

Akagi, T

Haruta, M

Akita, J

Nishida, A

Honda, Y

Takahashi, M ^{[1
]}

机构：

[1] Kyoto Univ Hosp, Translat Res Ctr, Dept Expt Therapeut, Kyoto 6068507, Japan

[2] Kyoto Univ Hosp, Grad Sch Med, Dept Ophthalmol & Visual Sci, Kyoto 6068507, Japan

[3] Tenri Yorozu Hosp, Dept Ophthalmol, Nara 6328552, Japan

[4] Osaka Biosci Inst, Dept Dev Biol, Osaka 5650874, Japan

来源：

NEUROSCIENCE LETTERS | 2003年 / 341卷 / 03期

关键词：

retinal cell culture; retinal transplantation; photoreceptor cells; retinal progenitor; retinal degeneration; culture period;

D O I：

10.1016/S0304-3940(03)00177-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Embryonic retina is one of the possible cell sources that will repair degenerated retina such as retinitis pigmentosa. Retinal progenitor cells isolated from embryonic rats could be cultured and expanded in serum free medium with both epidermal growth factor and basic fibroblast growth factor. We analyzed the properties of two different retinal progenitor cells in terms of culture periods. Retinal progenitor cells from embryonic retina could be expanded keeping immature cell properties and had the ability to migrate into degenerated adult retina from subretinal space after transplantation. They differentiated into neurons and glias, even into photoreceptor cells both in vitro and in vivo. However, they appeared to lose their tissue specificity after a long-term culture. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：213 / 216

页数：4

共 19 条

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