Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1-FFT cDNAs

被引：56

作者：

Hellwege, EM ^{[1
]}

Raap, M ^{[1
]}

Gritscher, D ^{[1
]}

Willmitzer, L ^{[1
]}

Heyer, AG ^{[1
]}

机构：

[1] Max Planck Inst Mol Pflanzenphysiol, D-14476 Golm, Germany

来源：

FEBS LETTERS | 1998年 / 427卷 / 01期

关键词：

fructan fructan 1-fructosyltransferase; inulin; fructan pattern; artichoke; Jerusalem artichoke;

D O I：

10.1016/S0014-5793(98)00386-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A newly isolated cDNA clone, Cy3, encoding the fructan fructan 1-fructosyltransferase (1-FFT) from artichoke was expressed using tobacco protoplasts as expression system, Analysis of the inulin molecules synthesized upon incubation of protoplast extracts with a mixture of oligofructans (DP3-5) shows the production of inulins with a degree of polymerization (DP) of up to 23, whereas parallel experiments performed using a 1-FFT cDNA from Jerusalem artichoke led to the production of fructans with a DP of up to only 12. The results of in vitro fructan synthesis catalyzed by transiently expressed enzymes therefore reflect the difference of in vivo fructan composition of Jerusalem artichoke (M-DP = 8-10) and artichoke (M-DP = 65). These data suggest that the fructan pattern in a given species is mainly defined by the enzymatic characteristics of 1-FFT, (C) 1998 Federation of European Biochemical Societies.

引用

页码：25 / 28

页数：4

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