Effect of high concentrations of bile acids on cultured hepatocytes

被引：12

作者：

Noto, H ^{[1
]}

Matsushita, M ^{[1
]}

Koike, M ^{[1
]}

Takahashi, M ^{[1
]}

Matsue, H ^{[1
]}

Kimura, J ^{[1
]}

Todo, S ^{[1
]}

机构：

[1] Hokkaido Univ, Sch Med, Dept Surg 1, Kita Ku, Sapporo, Hokkaido 060, Japan

来源：

ARTIFICIAL ORGANS | 1998年 / 22卷 / 04期

关键词：

bile acids; rat hepatocyte; C/CDC ratio; hepatocellular injury;

D O I：

10.1046/j.1525-1594.1998.05071.x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

High concentrations of bile acids have been reported as injurious to hepatocytes, the report the influence of various combinations of bi:le acids on the liver-specific function of cultured rat hepatocytes. Using 4 bile acids (glycocholate [GC], taurocholate [TC], glycohenodeoxycholate [GCDC], and taurochenodeoxycholate [TCDC]), we obtained 6 bile-acid mixtures, each; containing equal amounts of 2 bile acids (total bile acids [TBA], 2 mM). Changes in gluconeogenesis, ureagenesis, DNA contents, medium alanine aminotransferase, and morphologies were compared among the paired bile acid compositions by measuring the C/CDC ratio ([GC + TC]/[GCDC + TCDC]) of each. In terms of their relative impairments of ureagenesis from greatest to least, the acids were GCDC, TCDC, and GC, which was almost the same as TC. When the C/CDC ratio was 0, the values of all parameters measured deteriorated. When the C/CDC ratio was I in the presence of 1 mM GCDC, only the rate of ureagenesis was diminished. When the C/CDC ratio was infinite, no hepa to cellular injury was observed. GCDC and TCDC, together or separately, showed significant hepatocellular injury when the TEA concentration was 2 mM.

引用

页码：300 / 307

页数：8