Functional analysis of the Numb phosphotyrosine-binding domain using site-directed mutagenesis

The Numb protein is involved in cell fate determination during Drosophila neural development. Numb has a protein domain homologous to the phosphotyrosine-binding domain (PTB) in the adaptor protein She. In She, this domain interacts with specific phosphotyrosine containing motifs on receptor tyrosine kinases and other signaling molecules. Residues N-terminal to the phosphotyrosine are also crucial for phosphopeptide binding to the She PTB domain. Several amino acid residues in She have been implicated by site-directed mutagenesis to be critical for She binding to receptor tyrosine kinases, We have generated homologous mutations in Numb to test whether, in vivo, these changes affect Numb function during Drosophila sensory organ development, Two independent amino acid changes that interfere with She binding to phosphotyrosine residues do not affect Numb activity in vivo, In contrast, a mutation shown to abrogate the ability of the She PTB domain to bind residues upstream of the phosphotyrosine virtually eliminates Numb function. Similar results were observed in vitro by examining the binding of the Numb PTB domain to proteins from Schneider S2 cells. Our data confirm the importance of the PTB domain for Numb function but strongly suggest that the Numb PTB domain is not involved in phosphotyrosine-dependent interactions.