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Ultrastructural localization of the receptor for leptin in the rat hypothalamus

被引：14
作者：
Funahashi, H [1 ]
Ryushi, T
Mizushima, H
Katoh, S
Shioda, S
机构：
[1] Showa Univ, Sch Med, Dept Anat, Tokyo 1428555, Japan
[2] Tokyo Metropolitan Univ, Grad Sch Sci, Dept Kinesiol, Tokyo 1920364, Japan
来源：
HORMONES AND BEHAVIOR | 2000年 / 37卷 / 04期
关键词：
obesity; leptin receptor; immunocytochemistry; hypothalamus; rat;
D O I：
10.1006/hbeh.2000.1583
中图分类号：
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号：
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要：
Ultrastructural localization of the leptin receptor in the rat hypothalamus was studied by immunocytochemistry. The antiserum against the leptin receptor which was used specifically recognized the carboxy terminal of the cytoplasmic domain. Intense leptin receptor immunoreactivity was detected in the arcuate, paraventricular, and ventromedial nuclei of the hypothalamus and in the lateral hypothalamic area. At the ultrastructural level, leptin receptor-like immunoreactivity appeared to be concentrated predominantly in perikarya and dendrites of these areas and strong immunolabeling for the leptin receptor was detected in the plasma membrane, rough endoplasmic reticulum, Golgi apparatus, and cytoplasmic matrix. This study provides the first detailed fine structure of leptin receptor-immunoreactive neurons in the rat hypothalamus. It may help to provide better understanding of the functions of leptin in the rat hypothalamus. (C) 2000 Academic Press.
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页码：327 / 334
页数：8
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