Macromolecular crowding in biological systems:: hydrodynamics and NMR methods

被引:43
作者
Bernadó, P
de la Torre, JG
Pons, M
机构
[1] Univ Barcelona, Dept Quim Organ, E-08028 Barcelona, Spain
[2] Lab Biomol NMR, E-08028 Barcelona, Spain
[3] Univ Murcia, Fac Quim, Dept Quim Fis, E-30071 Murcia, Spain
关键词
NMR; rotational diffusion; translational diffusion; NMR relaxation; protein-protein interactions; macromolecular crowding;
D O I
10.1002/jmr.694
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Most biologically relevant environments involve highly concentrated macromolecular solutions and most biological processes involve macromolecules that diffuse and interact with other macromolecules. Macromolecular crowding is a general phenomenon that strongly affects the transport properties of macromolecules (rotational and translational diffusion) as well as the position of their equilibria. NMR methods can provide information on molecular interactions, as well as on translational and rotational diffusion. In fact, rotational diffusion, through its determinant role in NMR relaxation, places a practical limit on the systems that can be studied by NMR. While in dilute solutions of non-aggregating macromolecules this limit is set by macromolecular size, in crowded solutions excluded volume effects can have a strong effect on the observed diffusion rates. Hydrodynamic theory offers some insight into the magnitude of crowding effects on NMR observable parameters. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码:397 / 407
页数:11
相关论文
共 78 条
[41]   Evidence of red cell alignment in the magnetic field of an NMR spectrometer based on the diffusion tensor of water [J].
Kuchel, PW ;
Durrant, CJ ;
Chapman, BE ;
Jarrett, PS ;
Regan, DG .
JOURNAL OF MAGNETIC RESONANCE, 2000, 145 (02) :291-301
[42]   HETERONUCLEAR DOUBLE-QUANTUM-COHERENCE SELECTION WITH MAGNETIC-FIELD GRADIENTS IN DIFFUSION EXPERIMENTS [J].
KUCHEL, PW ;
CHAPMAN, BE .
JOURNAL OF MAGNETIC RESONANCE SERIES A, 1993, 101 (01) :53-59
[43]   MODEL-FREE APPROACH TO THE INTERPRETATION OF NUCLEAR MAGNETIC-RESONANCE RELAXATION IN MACROMOLECULES .2. ANALYSIS OF EXPERIMENTAL RESULTS [J].
LIPARI, G ;
SZABO, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (17) :4559-4570
[44]   MODEL-FREE APPROACH TO THE INTERPRETATION OF NUCLEAR MAGNETIC-RESONANCE RELAXATION IN MACROMOLECULES .1. THEORY AND RANGE OF VALIDITY [J].
LIPARI, G ;
SZABO, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (17) :4546-4559
[45]   MYOGLOBIN DIFFUSION IN BOVINE HEART-MUSCLE [J].
LIVINGSTON, DJ ;
LAMAR, GN ;
BROWN, WD .
SCIENCE, 1983, 220 (4592) :71-73
[46]   A NOVEL FLUORESCENCE RATIOMETRIC METHOD CONFIRMS THE LOW SOLVENT VISCOSITY OF THE CYTOPLASM [J].
LUBYPHELPS, K ;
MUJUMDAR, S ;
MUJUMDAR, RB ;
ERNST, LA ;
GALBRAITH, W ;
WAGGONER, AS .
BIOPHYSICAL JOURNAL, 1993, 65 (01) :236-242
[47]   PHYSICAL-PROPERTIES OF CYTOPLASM [J].
LUBYPHELPS, K .
CURRENT OPINION IN CELL BIOLOGY, 1994, 6 (01) :3-9
[48]   DIFFUSION OF A SMALL MOLECULE IN THE CYTOPLASM OF MAMMALIAN-CELLS [J].
MASTRO, AM ;
BABICH, MA ;
TAYLOR, WD ;
KEITH, AD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (11) :3414-3418
[49]   EFFECT OF MACROMOLECULAR CROWDING UPON THE STRUCTURE AND FUNCTION OF AN ENZYME - GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE [J].
MINTON, AP ;
WILF, J .
BIOCHEMISTRY, 1981, 20 (17) :4821-4826
[50]   Modeling of self-diffusion and relaxation time NMR in multi-compartment systems [J].
Novikov, EG ;
van Dusschoten, D ;
Van As, H .
JOURNAL OF MAGNETIC RESONANCE, 1998, 135 (02) :522-528