High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates

被引:243
作者
Simpson, PC
Roach, D
Woolley, AT
Thorsen, T
Johnston, R
Sensabaugh, GF
Mathies, RA
机构
[1] Univ Calif Berkeley, Coll Chem, Dept Chem, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Sch Publ Hlth, Forens Sci Grp, Berkeley, CA 94720 USA
[3] Mol Dynam, Sunnyvale, CA 94086 USA
关键词
D O I
10.1073/pnas.95.5.2256
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Capillary array electrophoresis (CAE) microplates that can analyze 96 samples in less than 8 min have been produced by bonding 10-cm-diameter micromachined glass wafers to form a glass sandwich structure. The microplate has 96 sample wells and 48 separation channels with an injection unit that permits the serial analysis of two different samples on each capillary, An elastomer sheet with an 8 by 12 array of holes is placed on top of the glass sandwich structure to define the sample wells, Samples are addressed with an electrode array that makes up the third layer of the assembly, Detection of all lanes with high temporal resolution was achieved by using a laser-excited confocal fluorescence scanner, To demonstrate the functionality of these microplates, electrophoretic separation and fluorescence detection of a restriction fragment marker for the diagnosis of hereditary hemochromatosis were performed, CAE microplates will facilitate all types of high-throughput genetic analysis because their high assay speed provides a throughput that is 50 to 100 times greater than that of conventional slab gels.
引用
收藏
页码:2256 / 2261
页数:6
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