Production and purification of α-amylase from hydrogen producing Enterobacter cloacae IIT-BT 08

被引：23

作者：

Kumar, N ^{[1
]}

Das, D ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

来源：

BIOPROCESS ENGINEERING | 2000年 / 23卷 / 02期

关键词：

Hydrogen; Purification; Fermentation; Starch; Incubation Time;

D O I：

10.1007/PL00009123

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enterobacter cloacae IIT-BT08 was found to produce both oc-amylase and hydrogen in a batch system using soluble starch as substrate. Incubation time, temperature, pH and substrate concentration for the maximum alpha-amylase activity (130 U/ml) were 8 h, 37 degrees C, 6.00 and 10 g/l of soluble potato starch respectively. However, the optimum temperature and ph for the crude cc-amylase activity were 60 degrees C and 4 respectively. The maximum rate of hydrogen production was observed at 10th h of fermentation and corresponding hydrogen yield was 7.6 mmol H-2/g soluble potato starch.

引用

页码：205 / 208

页数：4

共 19 条

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