Improved hydrophilic interaction chromatography method for the identification and quantification of glucosinolates

被引：52

作者：

Wade, Kristina L.

Garrard, Ian J.

Fahey, Jed W.

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Lewis B & Dorothy Cullman Canc Chemoprotect Ctr, Baltimore, MD 21205 USA

[2] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England

[3] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Int Hlth, Ctr Human Nutr, Baltimore, MD USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1154卷 / 1-2期

关键词：

glucoraphanin; broccoli; glucoiberin; chemoprotection; phytochemical; HPLC; sulforaphane glucosinolate;

D O I：

10.1016/j.chroma.2007.04.034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved hydrophilic interaction liquid chromatography (HILIC) method has been developed to separate members of a closely related family of chemoprotective phytochemicals called glucosinolates. This method exploits the emergence of a second generation of HILIC chemistry, using a silica-based permanently zwitterionic stationary phase. These columns are more robust, durable, and glucosinolates separations are more reproducible than with the original polyhydroxyethyl aspartamide columns. Furthermore, the HILIC system that we report herein permits much greater alteration of the mobile phase composition for customized separation of glucosinolates from plant extracts, across a wide spectrum of polarity. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：469 / 472

页数：4

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