Comparison of supercritical, subcritical, hot, pressurized and cold solvent extraction of four drugs from rodent food

Propranolol, tamoxifen and two experimental drugs (all basic but of differing pK(a) and polarity) were extracted from rodent food by supercritical carbon dioxide-methanol more efficiently than by a more polar, subcritical mixture of carbon dioxide and methanol, Hot, pressurized methanol alone was as efficient as the supercritical fluid, The efficiency of these methods was similar to conventional solvent extraction with acidified methanol, but conventional solvent extraction gave the best precision, as measured by the relative standard deviation.