PCR methods for rapid identification and characterization of Actinobacillus seminis strains

被引:20
作者
Appuhamy, S
Coote, JG
Low, JC
Parton, R
机构
[1] Univ Glasgow, Div Infect & Immun, Glasgow G12 8QQ, Lanark, Scotland
[2] Scottish Agr Coll, Vet Serv, Penicuik EH26 0QE, Midlothian, Scotland
关键词
D O I
10.1128/JCM.36.3.814-817.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced the simplest pattern and could be useful for identification of A. seminis and for its differentiation from related species. REP-and ERIC-PCR could be used for strain differentiation in epidemiological studies of A. seminis.
引用
收藏
页码:814 / 817
页数:4
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