Amperometric immunosensors based on protein A coupled polyaniline-perfluorosulfonated ionomer composite electrodes

被引：52

作者：

Liu, CH ^{[1
]}

Liao, KT ^{[1
]}

Huang, HJ ^{[1
]}

机构：

[1] Natl Sun Yat Sen Univ, Dept Chem, Kaohsiung 80424, Taiwan

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 13期

关键词：

D O I：

10.1021/ac9914317

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A very sensitive immunosensor based on polyaniline/Nafion/protein A (PA/NF/PrA) composite electrodes has been developed for the amperometric inmunoanalysis with urease-labeled immunoreagents. The use of urease conjugated goat anti-RIgG (GaRIgG-Ur) as the labeled antibody and urea as the substrate with an amperometric detection at -200 mV (vs Ag/AgCl) resulted in a dynamic range of 50-2000 ng mL(-1) and a low detection limit of 10 ng/mL (64 pM) for the immunoanalysis of rabbit immunoglobulin G (RIgG). Because of the special affinity between protein A and RIgG, the PA/NF/PrA electrode can be regenerated repetitively by changing the pH of the buffer solutions. Characteristics of the PA/NF/PrA/RIgG immunosensor and optimal conditions for the competitive immunoanalysis of RIgG with FIA were studied.

引用

页码：2925 / 2929

页数：5

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