Pyrosequencing™ -: Sequence typing at the speed of light

被引：30

作者：

Diggle, MA ^{[1
]}

Clarke, SC ^{[1
]}

机构：

[1] Stobhill Gen Hosp, Scottish Meningococcus & Pneumococcus Reference L, Dept Microbiol, Glasgow G21 3EU, Lanark, Scotland

来源：

MOLECULAR BIOTECHNOLOGY | 2004年 / 28卷 / 02期

关键词：

nucleotide sequencing; bacterial typing; Pyrosequencing;

D O I：

10.1385/MB:28:2:129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing(TM), a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.

引用

页码：129 / 137

页数：9

共 55 条

[1] Mitochondrial sequence analysis for forensic identification using pyrosequencing technology
Andréasson, H
Asp, A
Alderborn, A
Gyllensten, U
Allen, M
[J]. BIOTECHNIQUES, 2002, 32 (01) : 124 - +
[2] STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES INDUCTION OF TRANSFORMATION BY A DESOXYRIBONUCLEIC ACID FRACTION ISOLATED FROM PNEUMOCOCCUS TYPE III
Avery, Oswald T.
MacLeod, Colin M.
McCarty, Maclyn
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1944, 79 (02) : 137 - 158
[3] BOUKE B, 1999, J PEDIAT GASTROENTER, V29, P376
[4] BOUKE B, 1999, J PEDIAT GASTROENTER, V29, P377
[5] Molecular techniques: divide or share
Boxer, M
[J]. JOURNAL OF CLINICAL PATHOLOGY, 2000, 53 (01) : 19 - 21
[6] SOME RECENT STUDIES ON THE COMPOSITION AND STRUCTURE OF NUCLEIC ACIDS
CHARGAFF, E
[J]. JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1951, 38 (03): : 41 - 59
[7] Nucleotide sequence-based typing of bacteria and the impact of automation
Clarke, SC
[J]. BIOESSAYS, 2002, 24 (09) : 858 - 862
[8] Multilocus sequence typing and porA gene sequencing differentiates strains of Neisseria meningitidis during case clusters
Clarke, SC
Diggle, MA
Edwards, GFS
[J]. BRITISH JOURNAL OF BIOMEDICAL SCIENCE, 2002, 59 (03) : 160 - 162
[9] Clarke SC, 2001, BRIT J BIOMED SCI, V58, P230
[10] Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis
Clarke, SC
Diggle, MA
Edwards, GFS
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) : 3066 - 3071

← 1 2 3 4 5 6 →