Pyrosequencing™ -: Sequence typing at the speed of light

被引：30

作者：

Diggle, MA ^{[1
]}

Clarke, SC ^{[1
]}

机构：

[1] Stobhill Gen Hosp, Scottish Meningococcus & Pneumococcus Reference L, Dept Microbiol, Glasgow G21 3EU, Lanark, Scotland

来源：

MOLECULAR BIOTECHNOLOGY | 2004年 / 28卷 / 02期

关键词：

nucleotide sequencing; bacterial typing; Pyrosequencing;

D O I：

10.1385/MB:28:2:129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing(TM), a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.

引用

页码：129 / 137

页数：9

共 55 条

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