Crystal growth of proteins, nucleic acids, and viruses in gels

被引：56

作者：

Lorber, Bernard ^{[1
]}

Sauter, Claude ^{[1
]}

Theobald-Dietrich, Anne ^{[1
]}

Moreno, Abel ^{[2
,3
]}

Schellenberger, Pascale ^{[4
,5
]}

Robert, Marie-Claire ^{[6
]}

Capelle, Bernard ^{[7
]}

Sanglier, Sarah ^{[8
]}

Potier, Noelle ^{[9
]}

Giege, Richard ^{[1
]}

机构：

[1] Univ Strasbourg, Architecture & React ARN, CNRS, IBMC, F-67084 Strasbourg, France

[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[3] Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico

[4] INRA, UMR Sante Vigne & Qual Vin 1131, UDS, Lab Virol & Vect, F-68021 Colmar, France

[5] CNRS, UPR2357, Inst Biol Mol Plantes, F-37084 Strasbourg, France

[6] LMCP, F-75252 Paris, France

[7] IMPMC, UMR 7590, F-75015 Paris, France

[8] Univ Strasbourg, LSMBO, IPHC DSA, CNRS,UMR 7178, F-67087 Strasbourg, France

[9] CNRS, UMR 7177, Inst Chim LC3, F-67008 Strasbourg, France

来源：

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY | 2009年 / 101卷 / 1-3期

关键词：

Protein; Nucleic acid; RNA; DNA; Virus; Crystallisation; Gel; Agarose; Silica; Lipid phase; Cellulose; Capillary tube; Counter-diffusion; X-RAY-DIFFRACTION; ANGLE NEUTRON-SCATTERING; LIPID-CONTAINING SYSTEMS; AGAROSE-GEL; SINGLE-CRYSTALS; CUBIC PHASES; COUNTER-DIFFUSION; LYSOZYME CRYSTAL; CRYSTALLIZATION MEDIA; MEMBRANE-PROTEINS;

D O I：

10.1016/j.pbiomolbio.2009.12.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Medium-sized single crystals with perfect habits and no defect producing intense and well-resolved diffraction patterns are the dream of every protein crystallographer. Crystals of biological macromolecules possessing these characteristics can be prepared within a medium in which mass transport is restricted to diffusion. Chemical gels (like polysiloxane) and physical gels (such as agarose) provide such an environment and are therefore suitable for the crystallisation of biological macromolecules. Instructions for the preparation of each type of gel are given to urge crystal growers to apply diffusive media for enhancing crystallographic quality of their crystals. Examples of quality enhancement achieved with silica and agarose gels are given. Results obtained with other substances forming gel-like media (such as lipidic phases and cellulose derivatives) are presented. Finally, the use of gels in combination with capillary tubes for counter-diffusion experiments is discussed. Methods and techniques implemented with proteins can also be applied to nucleic acids and nucleoprotein assemblies such as viruses. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：13 / 25

页数：13

共 140 条

[61] Effects of pressure on the crystallization and the solubility of proteins in agarose gel [J].