The tumbling reactor: a new reactor design for biotechnology - Cultivation of the phototrophic micro-alga Scenedesmus communis

被引：4

作者：

Gorenflo, V

Schulze, S

Braun, AM

Stahl, W

机构：

[1] Univ Munster, Inst Mikrobiol, D-48149 Munster, Germany

[2] Degussa AG, Fachbereich Verfahrenstech, D-63457 Hanau, Germany

[3] Univ Karlsruhe, Lehrstuhl Umweltmesstech, D-76128 Karlsruhe, Germany

[4] Univ Karlsruhe, Inst Mech Verfahrenstech & Mech, D-76128 Karlsruhe, Germany

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 01期

关键词：

D O I：

10.1023/A:1008807610296

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new system for the growth of mammalian cells was developed and its biotechnological suitability was proven by the sterile cultivation of the phototrophic micro-alga Scenedesmus communis, as an easy to cultivate model organism. For this purpose, the bioreactor was combined with a newly developed equipment for irradiation of large scale cultures of phototrophic micro-organisms. Cultivation was performed in a 40 liter scale at 25 degrees C and a pH of 6.0 to 6.5. The aeration gas consisted of 2% or 7% of CO2 in N-2. The aeration rate was V-gas = 19.6 L.h(-1), the stirring speed was n = 70 or 56 min(-1). The new reactor consists of 8 totally immersed fluorescent tubes located around a cylinder for bubble free aeration which is moving within the cultivation broth. Results of the cultivation reveal the suitability of this newly designed system for biotechnological use and its high scale-up potential.

引用

页码：55 / 61

页数：7