Radiation synthesis and characterization of protein stabilized gold nanoparticles

被引：41

作者：

Akhavan, A. ^{[2
]}

Kalhor, H. R. ^{[1
]}

Kassaee, M. Z. ^{[2
]}

Sheikh, N. ^{[3
]}

Hassanlou, M. ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Fac Biol Sci, Dept Biochem, Tehran, Iran

[2] Tarbiat Modares Univ, Dept Chem, Tehran, Iran

[3] Nucl Sci & Technol Res Inst, Radiat Applicat Res Sch, Tehran, Iran

来源：

CHEMICAL ENGINEERING JOURNAL | 2010年 / 159卷 / 1-3期

关键词：

Gold nanoparticles; Radiation; Protein; COLORIMETRIC ASSAY; ALBUMIN; GROWTH; SIZE;

D O I：

10.1016/j.cej.2010.02.010

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

Gold nanoparticles were synthesized in a single step and a facile procedure by gamma-irradiation using bovine serum albumin protein as a stabilizer. The results of UV-vis absorption spectroscopy, transmission electron microscopy and X-ray diffraction analyses indicated that spherical well-dispersed gold nanoparticles ranging from 2 to 7 nm were generated, depending on the irradiation doses. The conjugation of BSA to the gold nanoparticles was indicated using FTIR spectroscopy and dynamic light scattering. Moreover, the effect of irradiation on the BSA structure in the presence and absence of Au3+ was studied by SOS-PAGE analysis. It became evident that gold nanoparticles formation partially protected against the degradation and aggregation of BSA. Methyl tetrazolium (MTT) assay demonstrated that the generated gold nanoparticles are not cytotoxic to mammalian cells in cultures. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：230 / 235

页数：6

共 30 条

[1]

Radiation-induced synthesis of mono- and multi-metallic clusters and nanocolloids [J].