Extracellular vesicle isolation and characterization: toward clinical application

被引：886

作者：

Xu, Rong ^{[1
]}

Greening, David W. ^{[1
]}

Zhu, Hong-Jian ^{[2
]}

Takahashi, Nobuhiro ^{[3
,4
]}

Simpson, Richard J. ^{[1
,4
]}

机构：

[1] La Trobe Univ, Dept Biochem & Genet, La Trobe Inst Mol Sci, Melbourne, Vic, Australia

[2] Univ Melbourne, Dept Surg, Melbourne, Vic, Australia

[3] Tokyo Univ Agr & Technol, Grad Sch Agr, Dept Appl Biol Sci, Tokyo, Japan

[4] Tokyo Univ Agr & Technol, Global Innovat Res Org, Tokyo, Japan

来源：

JOURNAL OF CLINICAL INVESTIGATION | 2016年 / 126卷 / 04期

基金：

英国医学研究理事会;

关键词：

NANOPARTICLE TRACKING ANALYSIS; CELL-DERIVED EXOSOMES; DENSITY-GRADIENT SEPARATION; MULTICOLOR FLOW-CYTOMETRY; MEMBRANE-VESICLES; CANCER EXOSOMES; MULTIVESICULAR BODIES; PURIFIED EXOSOMES; PLASMA-MEMBRANE; LARGE ONCOSOMES;

D O I：

10.1172/JCI81129

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.

引用

页码：1152 / 1162

页数：11

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