Indispensability of the Escherichia coli carbonic anhydrases YadF and CynT in cell proliferation at a low CO2 partial pressure

被引：34

作者：

Hashimoto, M ^{[1
]}

Kato, J ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Dept Biol, Hachioji, Tokyo 1920397, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 04期

关键词：

Escherichia coli; carbonic anhydrase; essential gene;

D O I：

10.1271/bbb.67.919

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli. However, mutant strains lacking both of them grew at high CO2 concentrations (5%), where non-enzymatic mechanisms generate HCO3-. This suggests that these carbonic anhydrases are essential because they maintain HCO3- levels at ambient CO2 concentrations.

引用

页码：919 / 922

页数：4

共 12 条

[1] NEW GENTAMICIN-RESISTANCE AND LACZ PROMOTER-PROBE CASSETTES SUITABLE FOR INSERTION MUTAGENESIS AND GENERATION OF TRANSCRIPTIONAL FUSIONS [J].

BECKER, A ;

SCHMIDT, M ;

JAGER, W ;

PUHLER, A .

GENE, 1995, 162 (01) :37-39

[2] The complete genome sequence of Escherichia coli K-12 [J].

Blattner, FR ;

Plunkett, G ;

Bloch, CA ;

Perna, NT ;

Burland, V ;

Riley, M ;

ColladoVides, J ;

Glasner, JD ;

Rode, CK ;

Mayhew, GF ;

Gregor, J ;

Davis, NW ;

Kirkpatrick, HA ;

Goeden, MA ;

Rose, DJ ;

Mau, B ;

Shao, Y .

SCIENCE, 1997, 277 (5331) :1453-+

[3] Crystal structure of E-coli β-carbonic anhydrase, an enzyme with an unusual pH-dependent activity [J].

Cronk, JD ;

Endrizzi, JA ;

Cronk, MR ;

O'Neill, JW ;

Zhang, KYJ .

PROTEIN SCIENCE, 2001, 10 (05) :911-922

[4] A PHYSIOLOGICAL-ROLE FOR CYANATE-INDUCED CARBONIC-ANHYDRASE IN ESCHERICHIA-COLI [J].

GUILLOTON, MB ;

LAMBLIN, AF ;

KOZLIAK, EI ;

GERAMINEJAD, M ;

TU, C ;

SILVERMAN, D ;

ANDERSON, PM ;

FUCHS, JA .

JOURNAL OF BACTERIOLOGY, 1993, 175 (05) :1443-1451

[5] Functional diversity, conservation, and convergence in the evolution of the alpha-, beta-, and gamma-carbonic anhydrase gene families [J].

HewettEmmett, D ;

Tashian, RE .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 1996, 5 (01) :50-77

[6] Construction of mini-F plasmid vectors for plasmid shuffling in Escherichia coli [J].

Kato, J ;

Ikeda, H .

GENE, 1996, 170 (01) :141-142

[7]

Kozliak EI, 2000, EXS, V90, P547

[8] Carbonic anhydrase is essential for growth of Ralstonia eutropha at ambient CO2 concentrations [J].

Kusian, B ;

Sültemeyer, D ;

Bowien, B .

JOURNAL OF BACTERIOLOGY, 2002, 184 (18) :5018-5026

[9] PCR-mediated gene replacement in Escherichia coli [J].

Murphy, KC ;

Campellone, KG ;

Poteete, AR .

GENE, 2000, 246 (1-2) :321-330

[10] Prokaryotic carbonic anhydrases [J].

Smith, KS ;

Ferry, JG .

FEMS MICROBIOLOGY REVIEWS, 2000, 24 (04) :335-366

← 1 2 →