Nested PCR detection of genetically modified soybean in soybean flour, infant formula and soymilk

被引：13

作者：

Angonesi Brod, Fabio Cristiano ^{[1
]}

Ferrari, Cibele dos Santos ^{[1
]}

Valente, Luciana Lehmkuhl ^{[1
]}

Malsonnave Arisi, Ana Carolina ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Ctr Ciencias Agrarias, Dept Ciencias & Tecnol Alimentos, BR-88034001 Florianopolis, SC, Brazil

来源：

LWT-FOOD SCIENCE AND TECHNOLOGY | 2007年 / 40卷 / 04期

关键词：

Roundup Ready (TM) soybean; qualitative PCR; GMO;

D O I：

10.1016/j.lwt.2005.12.009

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Due to the Brazilian market introduction of the genetically modified (GM) crop Roundup Ready (TM) (RR) soybean, the ability to detect GM crops has become a legal necessity. In order to detect the presence of RR soybean, a polymerase chain reaction (PCR) amplification method was evaluated for the detection of RR in soybean mixtures and commercially available soy flour, infant formula and soymilk powder. To detect the presence of RR soybean, a nested PCR resulted in an amplicon of 169 bp, present for all soybean mixed samples containing 0.01-10% GM soybean and absent for 0% GM soybean. None of the analysed infant formulas showed a positive signal after the nested PCR; four out of six soy flour samples and 15 out of 25 soymilk powder samples were positive for the presence of RR soybean. Results show that the nested PCR method used is adequate to determine the presence of GM soybean in the presented products. (c) 2006 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.

引用

页码：748 / 751

页数：4

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