Host-cell-specific glycosylation of HIV-2 envelope glycoprotein

被引：23

作者：

Liedtke, S ^{[1
]}

Geyer, R ^{[1
]}

Geyer, H ^{[1
]}

机构：

[1] UNIV GIESSEN,INST BIOCHEM,D-35392 GIESSEN,GERMANY

来源：

GLYCOCONJUGATE JOURNAL | 1997年 / 14卷 / 07期

关键词：

glycoprotein; glycosylation; gp120; HIV; MALDI-TOF-MS;

D O I：

10.1023/A:1018577619036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neutral complex-type N-glycans of the envelope glycoprotein 120 of HIV-2, propagated in different host cells, display cell-type specific variations. In order to identify typical structural elements, glycans were analysed by gel filtration, by enzymic sequencing and, in part, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The characteristic substituents of di-tri-and tetraantennary carbohydrate units thus observed include N-acetyllactosamine repeats, bisecting N-acetylglucosamine and fucose linked to the chitobiose core as well as to N-acetyllactosamine antennae. Each glycoprotein preparation displayed a characteristic set of glycoforms.

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收藏

页码：785 / 793

页数：9

相关论文

共 26 条

[1] EFFECT OF SIALIC-ACID REMOVAL ON THE ANTIBODY-RESPONSE TO THE 3RD VARIABLE DOMAIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN [J].

BENJOUAD, A ;

MABROUK, K ;

GLUCKMAN, JC ;

FENOUILLET, E .

FEBS LETTERS, 1994, 341 (2-3) :244-250

[2] CARBOHYDRATE DETERMINANT NEUAC-GAL-BETA(1-4) OF N-LINKED GLYCANS MODULATES THE ANTIGENIC ACTIVITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GLYCOPROTEIN GP120 [J].

BOLMSTEDT, A ;

OLOFSSON, S ;

SJOGRENJANSSON, E ;

JEANSSON, S ;

SJOBLOM, I ;

AKERBLOM, L ;

HANSEN, JES ;

HU, SL .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :3099-3105

[3] ALTERED HOST RANGE OF HIV-1 AFTER PASSAGE THROUGH VARIOUS HUMAN CELL-TYPES [J].

CHENGMAYER, C ;

SETO, D ;

LEVY, JA .

VIROLOGY, 1991, 181 (01) :288-294

[4] FUNCTIONS OF HIV ENVELOPE GLYCANS [J].

FENOUILLET, E ;

GLUCKMAN, JC ;

JONES, IM .

TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (02) :65-70

[5] Biological properties of recombinant HIV envelope synthesized in CHO glycosylation-mutant cell lines [J].

Fenouillet, E ;

Miquelis, R ;

Drillien, R .

VIROLOGY, 1996, 218 (01) :224-231

[6] EVIDENCE FOR A FUNCTIONAL INTERACTION BETWEEN THE V1/V2 AND C4 DOMAINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN GP120 [J].

FREED, EO ;

MARTIN, MA .

JOURNAL OF VIROLOGY, 1994, 68 (04) :2503-2512

[7] GLYCOSYLATION OF THE ENVELOPE GLYCOPROTEIN FROM A POLYTROPIC MURINE RETROVIRUS IN 2 DIFFERENT HOST-CELLS [J].

GEYER, H ;

KEMPF, R ;

SCHOTT, HH ;

GEYER, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 193 (03) :855-862

[8] CARBOHYDRATE STRUCTURE OF MARBURG VIRUS GLYCOPROTEIN [J].

GEYER, H ;

WILL, C ;

FELDMANN, H ;

KLENK, HD ;

GEYER, R .

GLYCOBIOLOGY, 1992, 2 (04) :299-312

[9] Glycosylation of recombinant ancrod from Agkistrodon rhodostoma after expression in mouse epithelial cells [J].

Geyer, H ;

Jacobi, I ;

Linder, D ;

Stirm, S ;

Bialojan, S ;

Strube, KH ;

Geyer, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 237 (01) :113-127

[10] INTERFERENCE WITH HIV-INDUCED SYNCYTIUM FORMATION AND VIRAL INFECTIVITY BY INHIBITORS OF TRIMMING GLUCOSIDASE [J].

GRUTERS, RA ;

NEEFJES, JJ ;

TERSMETTE, M ;

DEGOEDE, REY ;

TULP, A ;

HUISMAN, HG ;

MIEDEMA, F ;

PLOEGH, HL .

NATURE, 1987, 330 (6143) :74-77