Coupling liquid chromatography to Orbitrap mass spectrometry

被引：186

作者：

Makarov, Alexander ^{[1
]}

Scigelova, Michaela ^{[1
]}

机构：

[1] Thermo Fisher Sci, D-28199 Bremen, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 25期

关键词：

Orbitrap; LC-MS; High-resolution mass spectrometry; HIGH-RESOLUTION; PEPTIDE IDENTIFICATION; PERFORMANCE EVALUATION; QUANTITATIVE-ANALYSIS; ACCURACY; MS; PROTEOMICS; PHOSPHORYLATION; DISSOCIATION; METABOLITES;

D O I：

10.1016/j.chroma.2010.02.022

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The Orbitrap mass analyzer has become a mainstream mass spectrometry technique. In addition to providing a brief introduction to the Orbitrap technology and its continuing development, this article reviews the most recent publications quoting the use of the Orbitrap detection for a variety of chromatographic separation techniques. Its coupling to reversed-phase liquid chromatography (LC) represents undoubtedly the most ubiquitous approach to both small molecule and proteomic analyses. Multi-dimensional LC separations have an important role to play in the proteomics applications while an ultra-high-pressure LC is more frequently encountered in the area of metabolomics and metabolite analysis. Recently, special chromatographic techniques such as hydrophilic interaction chromatography and its variations have also been also cited with the Orbitrap detection. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：3938 / 3945

页数：8

共 61 条

[1]

Abian J, 1999, J MASS SPECTROM, V34, P244, DOI 10.1002/(SICI)1096-9888(199904)34:4<244::AID-JMS775>3.0.CO

[2]

2-0

[3]

Quantitative-Qualitative Data Acquisition Using a Benchtop Orbitrap Mass Spectrometer [J].