Preparative chromatography of furosemide 1-O-acyl-glucuronide from urine using micronized Amberiite XAD-2 and its application to other 1-O-acyl-glucuronides

被引：6

作者：

Mizuma, T ^{[1
]}

Benet, LZ

Lin, ET

机构：

[1] Tokyo Univ Pharm & Life Sci, Dept Biopharmaceut, Tokyo 19203, Japan

[2] Univ Calif San Francisco, Dept Biopharmaceut Sci, San Francisco, CA 94143 USA

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 1998年 / 28卷 / 01期

关键词：

D O I：

10.1080/10826069808010125

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Furosemide 1-O-acyl glucuronide (Fgnd) was extracted from the urine following oral administration of furosemide. The crude Fgnd was applied to micronized Amberlite XAD-2 column (2.5 cm i.d. x 90 cm length, 75-500 mu m particle size). The purified Fgnd was identified by mass spectrometry and beta-glucuronidase treatment. This method was also applicable to the purification of glucuronide of tolmetin (nonsteroidal anti-inflammatory drug, NSAID), suggesting that it was applicable to the other NSAIDs, most of which were known to be metabolized to acyl glucuronides.

引用

页码：37 / 47

页数：11