Fsp1-Mediated Lineage Tracing Fails to Detect the Majority of Disseminating Cells Undergoing EMT

被引：36

作者：

Bornes, Laura ^{[1
]}

van Scheppingen, Roan Hugo ^{[1
]}

Beerling, Evelyne ^{[1
]}

Schelfhorst, Tim ^{[1
]}

Ellenbroek, Saskia Inge Johanna ^{[1
]}

Seinstra, Danielle ^{[1
,2
]}

van Rheenen, Jacco ^{[1
]}

机构：

[1] Netherlands Canc Inst, Oncode Inst, Div Mol Pathol, Amsterdam, Netherlands

[2] Amsterdam UMC, Locat VuMC, Dept Pathol, Amsterdam, Netherlands

来源：

CELL REPORTS | 2019年 / 29卷 / 09期

基金：

欧盟地平线“2020”; 欧洲研究理事会;

关键词：

EPITHELIAL-MESENCHYMAL TRANSITION; METASTASIS; CANCER; PLASTICITY;

D O I：

10.1016/j.celrep.2019.10.107

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Epithelial-to-mesenchymal transition (EMT) has long been thought to be crucial for metastasis. Recently a study challenged this idea by demonstrating that metastases were seeded by tumor cells that were not marked by an EMT lineage-tracing reporter on the basis of the expression of the mesenchymal marker fsp1. However, the results of this study and their interpretation are under debate. Here, we combine the lineage-tracing reporter with our real-time EMT-state reporter and show that the fsp1-based EMT lineage-tracing reporter does not mark all disseminating mesenchymal cells with metastatic potential. Our findings demonstrate that fsp1-mediated lineage tracing does not allow any conclusions about the requirement of EMT for metastasis. Instead our data are fully consistent with previous reports that EMT is not a binary phenomenon but rather a spectrum of cellular states.

引用

页码：2565 / +

页数：8

共 23 条

[1]

EMT Subtype Influences Epithelial Plasticity and Mode of Cell Migration [J].