Chip-based optical detection of DNA hybridization by means of nanobead labeling

被引：127

作者：

Reichert, J ^{[1
]}

Csáki, A ^{[1
]}

Köhler, JM ^{[1
]}

Fritzsche, W ^{[1
]}

机构：

[1] Inst Phys High Technol, D-07702 Jena, Germany

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 24期

关键词：

D O I：

10.1021/ac000567y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new scheme for the detection of molecular interactions based on optical readout of nanoparticle labels has been developed. Capture DNA probes were arrayed on a glass chip and incubated with nanoparticle-labeled target DNA probes, containing a complementary sequence. Binding events were monitored by optical means, using reflected and transmitted light for the detection of surface-bound nanoparticles. Control experiments exclude significant influence of nonspecific binding on the observed contrast. Scanning force microscopy revealed the distribution of nanoparticles on the chip surface.

引用

页码：6025 / 6029

页数：5