Clean and rapid liquid chromatographic technique for monitoring of oxytetracycline and sulphadimicline in milk without use of organic solvents

被引:12
作者
Furusawa, N [1 ]
机构
[1] Osaka City Univ, Grad Sch Human Life Sci, Osaka 5588585, Japan
关键词
column liquid chromatography; ultrasonic homogenizer; oxytetracycline and sulphadimidine; solvent-free sample preparation; milk;
D O I
10.1007/BF02492402
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A clean method without use of organic solvents has been developed for isolation and high-performance liquid chromatographic (HPLC) determination of oxytetracycline (OTC) and sulphadimidine (SDD) in cows milk Isolation is rapid and simple - homogenization with an inorganic acid solution by means of a handy ultrasonic homogenizer, which is easy-to-use and portable, followed by centrifugation. Reversed-phase HPLC was performed on a C-4 column, with 1.25 mmol L-1 succinic acid solution as mobile phase, and identification was by means of a photodiode-array detector. Separation of the analytes was achieved in less than 8 min. Significant linearity was established over the concentration range of 0.1 - 1.0 mug mL(-1) for both target compounds (r > 0.99, P < 0.01). Average recoveries of OTC and SDD (each spiked at 0.1 - 1.0 mug mL(-1)) were greater than or equal to 88.8, and inter- and intra-assay variability was less than or equal to 2.8%. The total time required for analysis of one sample was < 20 min. The limits of quantitation of the method (mu g mL(-1) in milk) were 0.044 for OTC and 0.023 for SDD. No organic solvent was used at any stage of the analysis.
引用
收藏
页码:317 / 320
页数:4
相关论文
共 38 条
  • [1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY OF 10 SULFONAMIDE ANTIBIOTICS - ANALYSIS IN MILK AT THE PPB LEVEL
    ABIAN, J
    CHURCHWELL, MI
    KORFMACHER, WA
    [J]. JOURNAL OF CHROMATOGRAPHY, 1993, 629 (02): : 267 - 276
  • [2] CHROMATOGRAPHIC METHODS FOR TETRACYCLINE ANALYSIS IN FOODS
    BARKER, SA
    WALKER, CC
    [J]. JOURNAL OF CHROMATOGRAPHY, 1992, 624 (1-2): : 195 - 209
  • [3] BATTO G, 1989, J PHARM BIOMED ANAL, V20, P321
  • [4] SPE and MSPD as pre-separation techniques for HPLC of tetracyclines in meat, milk and cheese
    Brandsteterova, E
    Kubalec, P
    Bovanova, L
    Simko, P
    Bednarikova, A
    Machackova, L
    [J]. ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 1997, 205 (04): : 311 - 315
  • [5] An original approach to determining traces of tetracycline antibiotics in milk and eggs by solid-phase extraction and liquid chromatography/mass spectrometry
    Bruno, F
    Curini, R
    Di Corcia, A
    Nazzari, M
    Pallagrosi, M
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (14) : 1365 - 1376
  • [6] Confirmation of multiple tetracycline residues in milk and oxytetracycline in shrimp by liquid chromatography particle beam mass spectrometry
    Carson, MC
    Ngoh, MA
    Hadley, SW
    [J]. JOURNAL OF CHROMATOGRAPHY B, 1998, 712 (1-2): : 113 - 128
  • [7] DETECTION OF ANTIBIOTIC RESIDUES IN CONSUMER MILK SUPPLIES IN NORTH-AMERICA USING THE CHARM TEST-II PROCEDURE
    COLLINSTHOMPSON, DL
    WOOD, DS
    THOMSON, IQ
    [J]. JOURNAL OF FOOD PROTECTION, 1988, 51 (08) : 632 - &
  • [8] *COX AL COMM, 1993, JOINT FAO WHO FOOD S, V3
  • [9] Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent
    Ding, XJ
    Mou, SF
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 897 (1-2) : 205 - 214
  • [10] Determination of sulfonamides by packed column supercritical fluid chromatography with atmospheric pressure chemical ionisation mass spectrometric detection
    Dost, K
    Jones, DC
    Davidson, G
    [J]. ANALYST, 2000, 125 (07) : 1243 - 1247