Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma

被引：66

作者：

Chao, Angel

Tsai, Chia-Lung

Wei, Pei-Chi

Hsueh, Swei

Chao, An-Shine

Wang, Chin-Jung

Tsai, Chi-Neu ^{[1
]}

Lee, Yun-Shien ^{[2
]}

Wang, Tzu-Hao

Lai, Chyong-Huey

机构：

[1] Chang Gung Univ, Grad Inst Clin Med Sci, Tao Yuan, Taiwan

[2] Ming Chuan Univ, Dept Biotechnol, Taipei, Taiwan

来源：

CANCER LETTERS | 2010年 / 291卷 / 01期

关键词：

Choriocarcinoma; Gestation; MicroRNA; Mole; Placenta; PARAFFIN-EMBEDDED TISSUES; TOTAL RNA; GENE-EXPRESSION; CARCINOMA; PROFILES; TARGETS; CANCERS; SAMPLES; TUMORS; CELLS;

D O I：

10.1016/j.canlet.2009.10.005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We compared microRNA profiles between choriocarcinoma and non-cancerous trophoblasts, and revealed that miR-199b was underexpressed in choriocarcinoma. By computational prediction and microarray studies, SET (protein phosphatase 2A inhibitor) was shown to be one of the target genes regulated by miR-199b. Ectopic expression of miR-199b inhibited endogenous SET protein levels and the activity of the luciferase reporter containing the 3'-UTR of SET. Further comparisons of formalin-fixed paraffin-embedded human choriocarcinoma, mole, and non-cancer trophoblast tissues confirmed the initial findings of low miR-199b expression and SET upregulation in choriocarcinomas, suggesting that microRNA-dysregulated SET protein may account for the rapid growth seen with choriocarcinomas. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

收藏

页码：99 / 107

页数：9

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