A high-throughput DNA extraction method for barley seed

被引：44

作者：

von Post, R

von Post, L

Dayteg, C

Nilsson, M

Forster, BP

Tuvesson, S ^{[1
]}

机构：

[1] Svalof Weibull AB, SE-26881 Svalov, Sweden

[2] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland

来源：

EUPHYTICA | 2003年 / 130卷 / 02期

关键词：

barley; seed; high-throughput; DNA extraction; marker assisted selection;

D O I：

10.1023/A:1022863006134

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was developed in particular for a microsatellite marker genetically linked to barley yellow mosaic virus resistance, but it can be applied to other markers of interest for barley breeding. The quick seed extraction protocol makes it possible to handle thousands of samples per day. Extraction of DNA from seed also facilitates transfer of plant material compared to the long-distance transfer of leaf samples.

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收藏

页码：255 / 260

页数：6

相关论文

共 13 条

[1]

CHEUNG WY, 1993, PCR METH APPL, V3, P69

[2] PRE-GERMINATION GENOTYPIC SCREENING USING PCR AMPLIFICATION OF HALF-SEEDS [J].

CHUNWONGSE, J ;

MARTIN, GB ;

TANKSLEY, SD .

THEORETICAL AND APPLIED GENETICS, 1993, 86 (06) :694-698

[3]

Dayteg C, 1998, PLANT ANIMAL GENOME, P39

[4] Rapid and informative assays for Yd2, the barley yellow dwarf virus resistance gene, based on the nucleotide sequence of a closely linked gene [J].

Ford, CM ;

Paltridge, NG ;

Rathjen, JP ;

Moritz, RL ;

Simpson, RJ ;

Symons, RH .

MOLECULAR BREEDING, 1998, 4 (01) :23-31

[5] Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex [J].

Graner, A ;

Streng, S ;

Kellermann, A ;

Schiemann, A ;

Bauer, E ;

Waugh, R ;

Pellio, B ;

Ordon, F .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (02) :285-290

[6] A CODOMINANT PCR-BASED MARKER FOR ASSISTED SELECTION OF DURABLE STEM RUST RESISTANCE IN BARLEY [J].

HORVATH, DP ;

DAHLEEN, LS ;

STEBBING, JA ;

PENNER, G .

CROP SCIENCE, 1995, 35 (05) :1445-1450

[7] Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth) [J].

Ivandic, V ;

Walther, U ;

Graner, A .

THEORETICAL AND APPLIED GENETICS, 1998, 97 (08) :1235-1239

[8] A rapid DNA extraction method for RFLP and PCR analysis from a single dry seed [J].

Kang, HW ;

Cho, YG ;

Yoon, UH ;

Eun, MY .

PLANT MOLECULAR BIOLOGY REPORTER, 1998, 16 (01) :90-90

[9] Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.) [J].

Mano, Y ;

Sayed-Tabatabaei, BE ;

Graner, A ;

Blake, T ;

Takaiwa, F ;

Oka, S ;

Komatsuda, T .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (6-7) :937-946

[10] Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease [J].

Paltridge, NG ;

Collins, NC ;

Bendahmane, A ;

Symons, RH .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (08) :1170-1177