PRE-GERMINATION GENOTYPIC SCREENING USING PCR AMPLIFICATION OF HALF-SEEDS

被引:128
作者
CHUNWONGSE, J
MARTIN, GB
TANKSLEY, SD
机构
[1] Department of Plant Breeding and Biometry, Cornell University, Ithaca, 14853-1902, NY
关键词
PCR; RAPDS; RFLPS; MARKER-ASSISTED SELECTION;
D O I
10.1007/BF00222658
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A simple and rapid PCR-based method has been developed for determining the genotype of seeds before germination. Single half-seeds of rice (Oryza sativa L.) and wheat (Triticum aestivum L. em. Thell.) were preincubated, without grinding, in an aqueous extraction buffer. The resulting supernatants were then used in polymerase chain reaction (PCR) with oligonucleotide primers corresponding to rice single-copy sequences or a wheat microsatellite repeat. PCR products of identical size were amplified using either the half-seed extract or DNA isolated from leaf tissue. The remnant half-seeds can be maintained in ordered arrays using microtiter plates allowing the recovery of selected genotypes. Pre-germination genotypic screening of seed populations as described in this report should be useful for a variety of applications in plant breeding and genetics studies.
引用
收藏
页码:694 / 698
页数:5
相关论文
共 24 条
  • [1] Bernatzky R., 1986, PLANT MOL BIOL REP, V4, P37, DOI [10.1007/BF02672487, DOI 10.1007/BF02672487]
  • [2] DIRECT AMPLIFICATION OF PLANT GENOMIC DNA FROM LEAF AND ROOT PIECES USING PCR
    BERTHOMIEU, P
    MEYER, C
    [J]. PLANT MOLECULAR BIOLOGY, 1991, 17 (03) : 555 - 557
  • [3] HALF-SEED ANALYSIS - RAPID CHROMATOGRAPHIC DETERMINATION OF THE MAIN FATTY-ACIDS OF SUNFLOWER SEED
    CONTE, LS
    LEONI, O
    PALMIERI, S
    CAPELLA, P
    LERCKER, G
    [J]. PLANT BREEDING, 1989, 102 (02) : 158 - 165
  • [4] Dellaporta S.L., 1983, PLANT MOL BIOL REP, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]
  • [5] Doyle JJ., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362
  • [6] A SIMPLE AND RAPID METHOD FOR THE PREPARATION OF PLANT GENOMIC DNA FOR PCR ANALYSIS
    EDWARDS, K
    JOHNSTONE, C
    THOMPSON, C
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (06) : 1349 - 1349
  • [7] Grist D. H., 1975, RICE
  • [8] Isolation of Plant DNA: A Fast, Inexpensive, and Reliable Method
    Guillemaut, Pierre
    Marechal-Drouard, Laurence
    [J]. PLANT MOLECULAR BIOLOGY REPORTER, 1992, 10 (01) : 60 - 65
  • [9] HASH CT, 1981, BARLEY GENET NEWSL, V11, P74
  • [10] SIMULTANEOUS AMPLIFICATION AND DETECTION OF SPECIFIC DNA-SEQUENCES
    HIGUCHI, R
    DOLLINGER, G
    WALSH, PS
    GRIFFITH, R
    [J]. BIO-TECHNOLOGY, 1992, 10 (04): : 413 - 417