Validation of the rat limb bud micromass test in the international ECVAM validation study on three in vitro embryotoxicity tests

被引：45

作者：

Spielmann, H

Genschow, E

Brown, NA

Piersma, AH

Verhoef, A

Spanjersberg, MQI

Huuskonen, H

Paillard, F

Seiler, A

机构：

[1] BfR, Ctr Documentat & Evaluat Alternat Methods Anim Ex, ZEBET, D-12277 Berlin, Germany

[2] Univ London St Georges Hosp, Sch Med, London SW17 0RE, England

[3] Natl Inst Publ Hlth & Environm, RIVM, NL-3720 BA Bilthoven, Netherlands

[4] Natl Publ Hlth Inst, KTL Finland, Kuopio, Finland

[5] Sanofi Synthelabo Rech, Porcheville, France

[6] BfR, Fed Inst Risk Assessment, Berlin, Germany

来源：

ATLA-ALTERNATIVES TO LABORATORY ANIMALS | 2004年 / 32卷 / 03期

关键词：

ECVAM; in vitro embryotoxicity test; in vitro toxicology; micromass; prediction model; prevalidation; rat embryo limb buds; validation;

D O I：

10.1177/026119290403200306

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A detailed report is presented on the performance of the rat limb bud micromass (MM) test in a European Centre for the Evaluation of Alternative Methods (ECVAM)-sponsored formal validation study on three in vitro tests for embryotoxicity. Twenty coded test chemicals, classified as non-embryotoxic, weakly embryotoxic or strongly embryotoxic on the basis of their in vivo effects on animals and/or humans, were tested in four laboratories. The outcome showed that the MM test is an experimentally validated test, which holds promise for use for identifying strongly embryotoxic chemicals, but which needs to be improved before it can be recommended for use for regulatory purposes.

引用

页码：245 / 274

页数：30

共 23 条

[11]

FLINT OP, 1986, HDB EXPT PHARM, V96, P231

[12]

Genschow E, 2000, IN VITRO MOL TOXICOL, V13, P51

[13] Validation of the embryonic stem cell test in the international ECVAM validation study on three in vitro embryotoxicity tests [J].

Genschow, E ;

Spielmann, H ;

Scholz, G ;

Pohl, I ;

Seiler, A ;

Clemann, N ;

Bremer, S ;

Becker, K .

ATLA-ALTERNATIVES TO LABORATORY ANIMALS, 2004, 32 (03) :209-244

[14] The ECVAM international validation study on in vitro embryotoxicity tests:: Results of the definitive phase and evaluation of prediction models [J].

Genschow, E ;

Spielmann, H ;

Scholz, G ;

Seiler, A ;

Brown, N ;

Piersma, A ;

Brady, M ;

Clemann, N ;

Huuskonen, H ;

Paillard, F ;

Bremer, S ;

Becker, K .

ATLA-ALTERNATIVES TO LABORATORY ANIMALS, 2002, 30 (02) :151-176

[15]

Holzhutter HG, 1996, ATLA-ALTERN LAB ANIM, V24, P511

[16] LIMB BUD CELL-CULTURES FOR ESTIMATING THE TERATOGENIC POTENTIAL OF COMPOUNDS - VALIDATION OF THE TEST SYSTEM WITH RETINOIDS [J].

KISTLER, A .

ARCHIVES OF TOXICOLOGY, 1987, 60 (06) :403-414

[17] TERATOGENICITY OF AROTINOIDS (RETINOIDS) INVIVO AND INVITRO [J].

KISTLER, A ;

TSUCHIYA, T ;

TSUCHIYA, M ;

KLAUS, M .

ARCHIVES OF TOXICOLOGY, 1990, 64 (08) :616-622

[18]

Norusis M.J., 1994, Spss professional statistics 6.1

[19] Validation of the postimplantation rat whole-embryo culture test in the international ECVAM validation study on three in vitro embryotoxicity tests [J].

Piersma, AH ;

Genschow, E ;

Verhoef, A ;

Spanjersberg, MQI ;

Brown, NA ;

Brady, M ;

Burns, A ;

Clemann, N ;

Seiler, A ;

Spielmann, H .

ATLA-ALTERNATIVES TO LABORATORY ANIMALS, 2004, 32 (03) :275-307

[20] Reproduction and development [J].

Spielmann, H .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1998, 106 :571-576

← 1 2 3 →