Identification of differentially expressed genes in normal and tumor human gastric tissue

被引：80

作者：

Jung, MH ^{[1
]}

Kim, SC ^{[1
]}

Jeon, GA ^{[1
]}

Kim, SH ^{[1
]}

Kim, Y ^{[1
]}

Choi, KS ^{[1
]}

Park, SI ^{[1
]}

Joe, MK ^{[1
]}

Kimm, K ^{[1
]}

机构：

[1] Natl Inst Hlth, Dept Biomed Res, Div Canc Res, Eunpyung Ku, Seoul 122701, South Korea

来源：

GENOMICS | 2000年 / 69卷 / 03期

关键词：

D O I：

10.1006/geno.2000.6338

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The search for differentially expressed genes in gastric cancer may help define molecular alterations and molecular diagnosis of gastric cancer, Using the differential display PCR technique, we identified 18 genes that are differentially expressed between normal and tumor human gastric tissues, Their expressions were verified with reverse Northern blot analysis and Northern blot analysis, Oxidative phosphorylation-related genes, antizyme inhibitor of ornithine decarboxylase, protein phosphatase-1 beta, 35-kDa peroxisomal membrane protein, and cystic fibrosis transmembrane conductance receptor were highly expressed in tumor tissue, whereas pepsinogen A, Na-K ATPase cy subunit, nerve growth factor receptor, and cu-tropomyosin were highly expressed in normal tissue. In addition, 3 unknown genes were found to be differentially expressed in paired gastric tissues. These differentially expressed genes may provide significant opportunities for further understanding of gastric carcinogenesis and the molecular diagnosis of gastric cancer. (C) 2000 Academic Press.

引用

页码：281 / 286

页数：6

共 38 条

[31]

SHANTZ LM, 1994, CANCER RES, V54, P2313

[32]

Tahara E, 1996, SEMIN ONCOL, V23, P307

[33]

TAHARA E, 1995, CANCER, V75, P475

[34]

TORRONI A, 1990, J BIOL CHEM, V265, P20589

[35] Amplification of the c-met, c-erbB2 and epidermal growth factor receptor gene in human gastric cancers:: Correlation to clinical features [J].

Tsugawa, K ;

Yonemura, Y ;

Hirono, Y ;

Fushida, S ;

Kaji, M ;

Miwa, K ;

Miyazaki, I ;

Yamamoto, H .

ONCOLOGY, 1998, 55 (05) :475-481

[36]

Wang FL, 1996, CANCER RES, V56, P3634

[37] EVALUATION OF ORNITHINE DECARBOXYLASE ACTIVITY AS A MARKER FOR TUMOR-GROWTH RATE IN MALIGNANT-TUMORS [J].

WESTIN, T ;

EDSTROM, S ;

LUNDHOLM, K ;

GUSTAFSSON, B .

AMERICAN JOURNAL OF SURGERY, 1991, 162 (04) :288-293

[38]

YOSHIDA M, 1992, CANCER RES, V52, P6671

← 1 2 3 4 →