Cell adhesion to the extracellular matrix influences many cellular functions. The integrin family of matrix receptors plays major roles in the formation of adhesions, but other proteins modulate integrin signaling. Syndecan-4, a transmembrane proteoglycan, cooperatively signals with integrins during the formation of focal adhesions. To date, a direct link between syndecan-4 and the cytoskeleton has remained elusive. We now demonstrate by Triton X-100 extraction immunoprecipitation and in vitro binding assays that the focal adhesion component alpha-actinin interacts with syndecan-4 in a beta-integrin-independent manner.
机构:
Univ Paris 06, CNRS, Lab Biol Cellulaire Dev, UMR 7622, F-75252 Paris 05, FranceUniv Paris 06, CNRS, Lab Biol Cellulaire Dev, UMR 7622, F-75252 Paris 05, France
机构:
Univ Paris 06, CNRS, Lab Biol Cellulaire Dev, UMR 7622, F-75252 Paris 05, FranceUniv Paris 06, CNRS, Lab Biol Cellulaire Dev, UMR 7622, F-75252 Paris 05, France