An invertebrate (molluscan) plasma protein that binds to vertebrate immunoglobulins and its potential for yielding false-positives in antibody-based detection systems

被引：10

作者：

Hahn, UK

Fryer, SE

Bayne, CJ

机构：

[1] Department of Zoology, Oregon State University, Corvallis

[2] Department of Zoology, Cordley Hall 3029, Oregon State University, Corvallis

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 1996年 / 20卷 / 01期

关键词：

Biomphalaria glabrata; lectin; plasma protein; ELISA; Immunoassays;

D O I：

10.1016/0145-305X(95)00035-R

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Commercial immunoassay kits that incorporate monoclonal antibodies are being used increasingly to screen tissues and plasma from invertebrates for the presence of antigens that are taken to be homologues of the vertebrate immunogen. We report the presence of a protein (BgL115r) in the plasma of Biomphalaria glabrata that binds to immunoglobulins from a variety of vertebrates. BgL115r (ca. 230 kD non-reduced, ca. 115 kD reduced on SDS-PAGE) binds to the F(ab')(2)-region of murine IgG. Binding could be inhibited with high concentrations of N-acetyl-D-glucosamine (GlcNAc). BgL115r was found to yield false positive signals in an ELISA based assay. Copyright (C) 1996 Elsevier Science Ltd.

引用

页码：39 / 50

页数：12

共 29 条

[1] EVIDENCE FOR THE EARLY APPEARANCE OF INTERLEUKINS AND TUMOR-NECROSIS-FACTOR IN THE PHYLOGENESIS OF VERTEBRATES [J].