Phylogenetic characterization of a new thermoacidophilic bacterium isolated from hot springs in Japan

被引:25
作者
Hiraishi, A
Inagaki, K
Tanimoto, Y
Iwasaki, M
Kishimoto, N
Tanaka, H
机构
[1] Toyohashi Univ Technol, Dept Ecol Engn, Toyohashi, Aichi 441, Japan
[2] Okayama Univ, Fac Agr, Dept Bioresources Chem, Okayama 700, Japan
[3] Kurashiki Sakuyo Univ, Fac Food Culture, Kurashiki, Okayama 71002, Japan
关键词
Alicyclobacillus; hot spring; phylogeny; 16S rDNA; thermoacidophiles;
D O I
10.2323/jgam.43.295
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Three strains of thermophilic-acidophilic bacteria isolated previously from different hot springs in Japan were characterized by molecular genetic methods. The strategy taken involved PCR amplification, sequencing and restriction pattern analysis of 16S rDNA, 16S-23S rDNA spacer polymorphism analysis and genomic DNA-DNA hybridization. A phylogenetic analysis based on 16S rDNA sequences showed that the new thermoacidophilic isolates formed a genetically coherent group at the species level and fell into a major cluster together with members of the genera Alicyclobacillus and Sulfobacillus with A. acidocaldarius and A. acidoterrestris as their closest relatives. The levels of binary sequence similarity between the isolates and the two Alicyclobacillus species were 97.6 to 97.9%, values considered low enough to warrant placement of the isolates in a distinct species of the genus Alicyclobacillus. The 16S rDNA restriction pattern analysis, but not 16S-23S rDNA spacer polymorphism analysis, was useful for differentiating the isolates from the established Alicyclobacillus species. DNA-DNA hybridization assays demonstrated a distinct phylogenetic position of our isolates as a genospecies within the genus Alicyclobacillus. On the basis of these results, the thermoacidophilic isolates should be classified into a new species ef Alicyclobacillus. The results of this study suggest that this new genospecies of Alicyclobacillus is widely distributed in hot springs in Japan.
引用
收藏
页码:295 / 304
页数:10
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