Gold Nanoparticles as Radiation Sensitizers in Cancer Therapy

被引:522
作者
Chithrani, Devika B. [1 ,2 ]
Jelveh, Salomeh [3 ]
Jalali, Farid [2 ]
van Prooijen, Monique [2 ]
Allen, Christine [4 ]
Bristow, Robert G. [3 ,5 ,6 ]
Hill, Richard P. [3 ,5 ,6 ]
Jaffray, David A. [1 ,2 ,5 ,6 ]
机构
[1] Univ Hlth Network, STTARR Innovat Ctr, Toronto, ON, Canada
[2] Univ Hlth Network, Radiat Med Program, Princess Margaret Hosp, Toronto, ON, Canada
[3] Univ Hlth Network, Ontario Canc Inst, Toronto, ON, Canada
[4] Univ Toronto, Leslie Dan Fac Pharm, Toronto, ON, Canada
[5] Univ Toronto, Dept Radiat Oncol, Toronto, ON, Canada
[6] Univ Toronto, Dept Med Biophys, Toronto, ON, Canada
关键词
DNA-REPAIR; DOSE ENHANCEMENT; CELLULAR UPTAKE; SIZE; CELLS; RADIOSENSITIZATION; NANOTECHNOLOGY; CYTOTOXICITY; RADIOTHERAPY; MECHANISM;
D O I
10.1667/RR1984.1
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Among other nanoparticle systems, gold nanoparticles have been explored as radiosensitizers. While most of the research in this area has focused on either gold nanoparticles with diameters of less than 2 nm or particles with micrometer dimensions, it has been shown that nanoparticles 50 nm in diameter have the highest cellular uptake. We present the results of in vitro studies that focus on the radiosensitization properties of nanoparticles in the size range from 14-74 nm. Radiosensitization was dependent on the number of gold nanoparticles internalized within the cells. Gold nanoparticles 50-nm in diameter showed the highest radiosensitization enhancement factor (REF) (1.43 at 220 kVp) compared to gold nanoparticles of 14 and 74 nm (1.20 and 1.26, respectively). Using 50-nm gold nanoparticles, the REF for lower- (105 kVp) and higher- (6 MVp) energy photons was 1.66 and 1.17, respectively. DNA double-strand breaks were quantified using radiation-induced foci of gamma-H2AX and 53BP1, and a modest increase in the number of foci per nucleus was observed in irradiated cell populations with internalized gold nanoparticles. The outcome of this research will enable the optimization of gold nanoparticle-based sensitizers for use in therapy. (C) 2010 by Radiation Research Society
引用
收藏
页码:719 / 728
页数:10
相关论文
共 52 条
[1]  
[Anonymous], 2003, WORLD CANC REPORT
[2]   Growth of gold nanoparticles in human cells [J].
Anshup ;
Venkataraman, JS ;
Subramaniam, C ;
Kumar, RR ;
Priya, S ;
Kumar, TRS ;
Omkumar, RV ;
John, A ;
Pradeep, T .
LANGMUIR, 2005, 21 (25) :11562-11567
[3]  
Banáth JP, 2003, CANCER RES, V63, P4347
[4]   Microscopic imaging of DNA repair foci in irradiated normal tissues [J].
Bhogal, Nirmal ;
Jalali, Farid ;
Bristow, Robert G. .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2009, 85 (09) :732-746
[5]   Hypoxia, DNA repair and genetic instability [J].
Bristow, Robert G. ;
Hill, Richard P. .
NATURE REVIEWS CANCER, 2008, 8 (03) :180-192
[6]   Parameters governing gold nanoparticle X-ray radiosensitization of DNA in solution [J].
Brun, Emilie ;
Sanche, Leon ;
Sicard-Roselli, Cecile .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2009, 72 (01) :128-134
[7]   Variation of strand break yield for plasmid DNA irradiated with high-Z metal nanoparticles [J].
Butterworth, K. T. ;
Wyer, J. A. ;
Brennan-Fournet, M. ;
Latimer, C. J. ;
Shah, M. B. ;
Currell, F. J. ;
Hirst, D. G. .
RADIATION RESEARCH, 2008, 170 (03) :381-387
[8]   Increased apoptotic potential and dose-enhancing effect of gold nanoparticles in combination with single-dose clinical electron beams on tumor-bearing mice [J].
Chang, Meng-Ya ;
Shiau, Ai-Li ;
Chen, Yu-Hung ;
Chang, Chih-Jui ;
Chen, Helen H-W ;
Wu, Chao-Liang .
CANCER SCIENCE, 2008, 99 (07) :1479-1484
[9]   Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes [J].
Chithrani, B. Devika ;
Chan, Warren C. W. .
NANO LETTERS, 2007, 7 (06) :1542-1550
[10]   Intracellular uptake, transport, and processing of nanostructures in cancer cells [J].
Chithrani, B. Devika ;
Stewart, James ;
Allen, Christine ;
Jaffray, David A. .
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2009, 5 (02) :118-127