Damage induced by stannous chloride in plasmid DNA

被引：47

作者：

de Mattos, JCP

Dantas, FJS

Bezerra, RJAC

Bernardo, M

Cabral-Neto, JB

Lage, C

Leitao, AC

Caldeira-de-Araújo, A

机构：

[1] Univ Estado Rio De Janeiro, Dept Biofis & Biometr, Inst Biol Roberto Alcantara Gomes, BR-20551030 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Lab Radiobiol Mol, IBCCF, CCS, BR-21949900 Rio De Janeiro, Brazil

来源：

TOXICOLOGY LETTERS | 2000年 / 116卷 / 1-2期

关键词：

stannous chloride; reactive oxygen species; DNA damage; plasmid DNA;

D O I：

10.1016/S0378-4274(00)00213-7

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Stannous chloride (SnCl2) is widely used in daily human life, for example, to conserve soft drinks, in food manufacturing and biocidal preparations. In nuclear medicine, stannous chloride is used as a reducing agent of Technetium-99m, a radionuclide used to label different cells and molecules. In spite of this, stannous chloride is able to generate reactive oxygen species (ROS) which can damage DNA. In this work, plasmid DNA (pUC 9.1) was incubated with SnCl2 under different conditions and the results analyzed through DNA migration in agarose gel electrophoresis. Our data reinforce the powerful damaging effect induced by stannous ion and suggest that this salt can play a direct role in inducing DNA lesions. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：159 / 163

页数：5

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