Simultaneous detection of different glycosidase activities by 19F NMR spectroscopy

被引：18

作者：

Albert, M

Repetschnigg, W

Ortner, J

Gomes, J

Paul, BJ

Illaszewicz, C

Weber, H

Steiner, W

Dax, K

机构：

[1] Graz Univ Technol, Inst Organ Chem, A-8010 Graz, Austria

[2] Graz Univ Technol, Inst Biotechnol, A-8010 Graz, Austria

来源：

CARBOHYDRATE RESEARCH | 2000年 / 327卷 / 04期

基金：

奥地利科学基金会;

关键词：

glycosyl fluorides; glycosidases; F-19 NMR spectroscopy; beta-D-mannopyranosyl fluoride;

D O I：

10.1016/S0008-6215(00)00060-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast method for the simultaneous detection of different glycosidolytic activities in commercially available enzyme preparations and crude culture filtrates was found in using, as substrate, a mixture of different glycosyl fluorides and F-19 NMR spectroscopy as a screening technique. Accompanying studies regarding the hydrolytic stability of these fluorides in various buffer systems, as well as conditions of their long-term storage, were carried out. A simple procedure for the preparation of beta-D-mannopyranosyl fluoride in gram quantities is given. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：395 / 400

页数：6

共 18 条

[1]

ALBERT M, 1998, 19 INT CARB S SAN DI

[2] HYDROLYSIS OF GLYCOSYL FLUORIDES BY GLYCOSIDASES [J].