Concise Review: Isoforms of OCT4 Contribute to the Confusing Diversity in Stem Cell Biology

被引：193

作者：

Wang, Xia ^{[1
]}

Dai, Jianwu ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100190, Peoples R China

来源：

STEM CELLS | 2010年 / 28卷 / 05期

关键词：

OCT4; OCT4A; OCT4B; OCT4B1; Alternative splicing; Alternative translation initiation; NUCLEAR-LOCALIZATION; RNA INTERFERENCE; PROSTATE-CANCER; SELF-RENEWAL; POU DOMAIN; GERM-CELLS; EXPRESSION; PLURIPOTENT; MARKER; OCT-3/4;

D O I：

10.1002/stem.419

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The human OCT4 gene can generate at least three transcripts (OCT4A, OCT4B, and OCT4B1) and four protein isoforms (OCT4A, OCT4B-190, OCT4B-265, and OCT4B-164) by alternative splicing and alternative translation initiation. OCT4A is a transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. While OCT4B cannot sustain ES cell self-renewal, it may respond to cell stresses. Yet, the function of OCT4B1 is still unclear. Lack of distinction of OCT4 isoforms could lead to confusions and controversies on OCT4 in various tissues and cells. One important issue we emphasize in this review article is that alternatively spliced transcripts and alternative translation products of OCT4 exhibit diverse expression patterns and functions. Furthermore, simple approaches and methods to detect and distinguish OCT4 isoforms are discussed. This article underscores the importance of identifying and discriminating the expression and functions of OCT4 isoforms in stem cell research. STEM CELLS 2010; 28: 885-893

引用

页码：885 / 893

页数：9

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