Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing

被引：180

作者：

Wilson, KS ^{[1
]}

Noller, HF ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA

来源：

CELL | 1998年 / 92卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(00)80905-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of translational elongation factor EF-G with the ribosome in the posttranslocational state has been mapped by directed hydroxyl radical probing. Localized hydroxyl radicals were generated from Fe(II) tethered to 18 different sites on the surface of EF-G bound to the ribosome. Cleavages in ribosomal RNA were mapped, providing proximity relationships between specific sites of EF-G and rRNA elements of the ribosome. Collectively, these data provide a set of constraints by which EF-G can be positioned unambiguously in the ribosome at low resolution. The proximities of different domains of EF-G to well-characterized elements of rRNA have additional implications for the mechanism of protein synthesis.

引用

页码：131 / 139

页数：9

共 51 条

[21] FUSIDIC ACID-RESISTANT MUTANTS DEFINE 3 REGIONS IN ELONGATION-FACTOR-G OF SALMONELLA-TYPHIMURIUM [J].

JOHANSON, U ;

HUGHES, D .

GENE, 1994, 143 (01) :55-59

[22] Mapping the inside of the ribosome with an RNA helical ruler [J].

Joseph, S ;

Weiser, B ;

Noller, HF .

SCIENCE, 1997, 278 (5340) :1093-1098

[23] PHOTOCHEMICAL CROSS-LINKING OF ELONGATION FACTOR-G TO 70-S RIBOSOMES FROM ESCHERICHIA-COLI BY 4-(6-FORMYL-3-AZIDOPHENOXY)BUTYRIMIDATE [J].

MAASSEN, JA ;

MOLLER, W .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 115 (02) :279-285

[24] IDENTIFICATION OF INTERMOLECULAR RNA CROSS-LINKS AT THE SUBUNIT INTERFACE OF THE ESCHERICHIA-COLI RIBOSOME [J].

MITCHELL, P ;

OSSWALD, M ;

BRIMACOMBE, R .

BIOCHEMISTRY, 1992, 31 (11) :3004-3011

[25] INTERMEDIATE STATES IN THE MOVEMENT OF TRANSFER-RNA IN THE RIBOSOME [J].

MOAZED, D ;

NOLLER, HF .

NATURE, 1989, 342 (6246) :142-148

[26] INTERACTION OF ELONGATION-FACTORS EF-G AND EF-TU WITH A CONSERVED LOOP IN 23S RNA [J].

MOAZED, D ;

ROBERTSON, JM ;

NOLLER, HF .

NATURE, 1988, 334 (6180) :362-364

[27] TRANSFER-RNA SHIELDS SPECIFIC NUCLEOTIDES IN 16S RIBOSOMAL-RNA FROM ATTACK BY CHEMICAL PROBES [J].

MOAZED, D ;

NOLLER, HF .

CELL, 1986, 47 (06) :985-994

[28] A new model for the three-dimensional folding of Escherichia coli 16 S ribosomal RNA .1. Fitting the RNA to a 3D electron microscopic map at 20 angstrom [J].

Mueller, F ;

Brimacombe, R .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 271 (04) :524-544

[29] CRYSTAL-STRUCTURE OF THE TERNARY COMPLEX OF PHE-TRNA(PHE), EF-TU, AND A GTP ANALOG [J].

NISSEN, P ;

KJELDGAARD, M ;

THIRUP, S ;

POLEKHINA, G ;

RESHETNIKOVA, L ;

CLARK, BFC ;

NYBORG, J .

SCIENCE, 1995, 270 (5241) :1464-1472

[30]

NOLLER HF, 1989, RIBOSOME STRUCTURE F, P73

← 1 2 3 4 5 6 →