Bat Coronaviruses and Experimental Infection of Bats, the Philippines

被引：153

作者：

Watanabe, Shumpei

Masangkay, Joseph S. ^{[2
]}

Nagata, Noriyo ^{[3
]}

Morikawa, Shigeru ^{[3
]}

Mizutani, Tetsuya ^{[3
]}

Fukushi, Shuetsu ^{[3
]}

Alviola, Phillip ^{[2
]}

Omatsu, Tsutomu ^{[3
]}

Ueda, Naoya

Iha, Koichiro

Taniguchi, Satoshi

Fujii, Hikaru

Tsuda, Shumpei

Endoh, Maiko

Kato, Kentaro

Tohya, Yukinobu ^{[4
]}

Kyuwa, Shigeru

Yoshikawa, Yasuhiro

Akashi, Hiroomi ^{[1
]}

机构：

[1] Univ Tokyo, Dept Vet Microbiol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Univ Philippines, Los Banos, Laguna, Philippines

[3] Natl Inst Infect Dis, Tokyo, Japan

[4] Nihon Univ, Kanagawa, Japan

来源：

EMERGING INFECTIOUS DISEASES | 2010年 / 16卷 / 08期

基金：

日本学术振兴会;

关键词：

VIRUS; TRANSCRIPTION; PREVALENCE; DIVERSITY;

D O I：

10.3201/eid1608.100208

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription-PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9-1/China/2007 (83% identity), respectively. To propagate group 2 Co Vs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection.

引用

页码：1217 / 1223

页数：7

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