Spectrophotometric determination of deacetylation degree of chitinous materials dissolved in phosphoric acid

被引：12

作者：

Hsiao, HY ^{[1
]}

Tsai, CC ^{[1
]}

Chen, SM ^{[1
]}

Hsieh, BC ^{[1
]}

Chen, RLC ^{[1
]}

机构：

[1] Natl Taiwan Univ, Biosensor Lab, Dept Bioind Mechatron Engn, Coll Bioresources & Agr, Taipei 106, Taiwan

来源：

MACROMOLECULAR BIOSCIENCE | 2004年 / 4卷 / 10期

关键词：

chitin; chitosan; deacetylation degree; NMR; UV-vis spectroscopy;

D O I：

10.1002/mabi.200400084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV-transparent solvent system. Calibrating by the extinction coefficients (A(210)) of D-glucosamine and N-acetyl-D-glucosamine, DD values (24-88%) were computed numerically. The results correlated well (R-2 = 0.9805 n = 50) with those obtained by solid-state C-13 NMR.

引用

收藏

页码：919 / 921

页数：3

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