In vivo electrically mediated protein and gene transfer in murine melanoma

被引：323

作者：

Rols, MP ^{[1
]}

Delteil, C ^{[1
]}

Golzio, M ^{[1
]}

Dumond, P ^{[1
]}

Cros, S ^{[1
]}

Teissie, J ^{[1
]}

机构：

[1] Inst Pharmacol & Struct Biol, UPR 9062, F-31062 Toulouse, France

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 02期

关键词：

drug delivery; gene therapy; electropermeabilization;

D O I：

10.1038/nbt0298-168

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We show that efficient permeabilization of murine melanoma can be obtained in vivo by applying electric pulses. More than 80% of the cell population is affected as shown by the penetration of propidium iodide. A protein, beta-galactosidase, can be transferred and expressed into the cells by incorporating either the protein or a plasmid carrying the reporter gene with respective efficiencies of 20% and 4%, This is obtained by a direct injection of either the protein or the plasmid in the tumor, followed by the application of electric pulses with surface electrodes in contact with the skin. This approach is simple and safe to use, reproducible, and specific; moreover, it is potentially applicable to a wide variety of tissues, cell types, and animals.

引用

页码：168 / 171

页数：4