Preparation and antibacterial activity of Fe3O4@Ag nanoparticles

被引:476
作者
Gong, Ping [1 ]
Li, Huimin [1 ]
He, Xiaoxiao [1 ]
Wang, Kemin [1 ]
Hu, Jianbing [1 ]
Tan, Weihong [1 ]
Zhang, Shouchun [1 ]
Yang, Xiaohai [1 ]
机构
[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Biomed Engn Ctr, Changsha 410082, Peoples R China
关键词
D O I
10.1088/0957-4484/18/28/285604
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Bifunctional Fe3O4@ Ag nanoparticles with both superparamagnetic and antibacterial properties were prepared by reducing silver nitrate on the surface of Fe3O4 nanoparticles using the water-in-oil microemulsion method. Formation of well-dispersed nanoparticles with sizes of 60 +/- 20 nm was confirmed by transmission electron microscopy and dynamic light scattering. X-ray diffraction patterns and UV-visible spectroscopy indicated that both Fe3O4 and silver are present in the same particle. The superparamagnetism of Fe3O4@Ag nanoparticles was confirmed with a vibrating sample magnetometer. Their antibacterial activity was evaluated by means of minimum inhibitory concentration value, flow cytometry, and antibacterial rate assays. The results showed that Fe3O4@Ag nanoparticles presented good antibacterial performance against Escherichia coli (gram-negative bacteria), Staphylococcus epidermidis (gram-positive bacteria) and Bacillus subtilis ( spore bacteria). Furthermore, Fe3O4@Ag nanoparticles can be easily removed from water by using a magnetic field to avoid contamination of surroundings. Reclaimed Fe3O4@Ag nanoparticles can still have antibacterial capability and can be reused.
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页数:7
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