Utilization of next-generation sequencing platforms in plant genomics and genetic variant discovery

被引:86
作者
Deschamps, Stephane [1 ]
Campbell, Matthew A. [2 ]
机构
[1] DuPont Crop Genet, Expt Stn, Wilmington, DE 19880 USA
[2] Pioneer HiBred Int Inc, Johnston, IA 50131 USA
关键词
Plant genomics; SNP discovery; Genome; Transcriptome; Resequencing; Next generation sequencing; SHORT DNA-SEQUENCES; POLYMORPHISM SNP DISCOVERY; WHOLE-GENOME; LARGE-SCALE; DIFFERENTIAL METHYLATION; DRAFT SEQUENCE; BASE CALLER; SINGLE; IDENTIFICATION; ALIGNMENT;
D O I
10.1007/s11032-009-9357-9
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Genome-wide variant detection within a species is the primary initial step towards linking genotypic variation and phenotypes. The conversion of these genetic variants (the most prevalent of these being single-nucleotide polymorphisms or SNPs) into genetic markers is particularly important in agronomically valuable crop species to allow for cost-effective marker-assisted selection strategies, whole-genome fingerprinting, association studies, map-based gene cloning and population-based analyses. Towards these goals, an increasing number of large-scale genetic variant discovery initiatives are being undertaken in conjunction with next-generation sequencing platforms, allowing for drastically quicker and cheaper variant discovery, and leading towards a far more comprehensive view of the genome or transcriptome. This review will summarize the current status of these initiatives and will discuss the expanding role of next-generation sequencing technologies in facilitating crop improvement.
引用
收藏
页码:553 / 570
页数:18
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