Detection of toxigenic Clostridium difficile in diarrheal stools by rapid real-time polymerase chain reaction

被引：27

作者：

Goldenberg, Simon D. ^{[1
,2
]}

Dieringer, Thomas ^{[3
]}

French, Gary L. ^{[1
,2
]}

机构：

[1] Guys & St Thomas NHS Fdn Trust, Directorate Infect, London SE1 7EH, England

[2] Kings Coll London, Sch Med, Div Immunol Infect & Inflammatory Dis, London SE1 1UL, England

[3] Univ Rochester, Rochester, NY USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 67卷 / 03期

关键词：

Clostridium difficile; Molecular diagnostics; PCR; INFECTION; DIAGNOSIS; CULTURE; GENE;

D O I：

10.1016/j.diagmicrobio.2010.02.019

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The Cepheid Xpert polymerase chain reaction assay (Sunnyvale, CA) had a sensitivity of 100%, specificity of 96.7%, and positive and negative predictive values of 90.5% and 100%, respectively, compared with toxigenic culture for the laboratory diagnosis of Clostridium difficile in diarrheal stool samples. This test appears to be a significant improvement to poorly performing enzyme immunoassays. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：304 / 307

页数：4

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